pPICZα A

5´ AOX1 promoter region: bases 1-941

5´ AOX1 priming site: bases 855-875

α-factor signal sequence: bases 941-1207

Multiple cloning site: bases 1208-1276

c-myc epitope: bases 1275-1304

Polyhistidine （6xHis） tag: bases 1320-1337

3´ AOX1 priming site: bases 1423-1443

AOX1 transcription termination region: bases 1341-1682

TEF1 promoter: bases 1683-2093

EM7 promoter: bases 2095-2162

Sh ble ORF: bases 2163-2537

CYC1 transcription termination region: bases 2538-2855

pUC origin: bases 2866-3539 （complementary strand）

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